Manufacturing and Properties of Poly Vinyl Alcohol/Fibrin Nanocomposite Used for Wound Dressing

  • Fahime Sadat Hoseini Shahrood University of Technology, Shahrood, Iran.
  • Reza Taherian Shahrood University of Technology, Shahrood, Iran.
  • Amir Atashi University of Medical Sciences, Shahrood, Iran.
Keywords: Adhesive; Biomaterial; Wound Treating; Electrospinning; Nanocomposites

Abstract

The purpose of this study is to make PVA/fibrin nanocomposite with suitable mechanical and biological properties to be used in wound dressing. Characterizations are performed by SEM, microculture Tetrazolium Test (MTT), tensile strength, contact angle, and DAPI coloring. Low, medium and high Concentrations of fibrinogen and thrombin were injected into the scaffolds by electrophoresis method. The tensile strength test of scaffolds shows that the ultimate tensile strength and fracture strain are 4.06 MPa and 0.93, respectively. Wettability and SEM tests show an increase in hydrophobicity by the addition of fibrin to the scaffolds. The DAPI coloring test or cell adhesion test also indicates an increase in cell adhesion of scaffolds contained fibrin in comparison to scaffolds without fibrin. MTT test for 4 days shows a better cell proliferation in the PVA/fibrin composites as compared to PVA scaffolds results Showed that PVA/fibrin nanocomposite could be used for tissue engineering applications as a wound dressing in animal models.

Author Biographies

Fahime Sadat Hoseini, Shahrood University of Technology, Shahrood, Iran.

Faculty of Materials Science & Engineering

Reza Taherian, Shahrood University of Technology, Shahrood, Iran.

Faculty of Materials Science & Engineering

Amir Atashi, University of Medical Sciences, Shahrood, Iran.

School of Medicine

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Published
2021-03-20
Section
Articles