Virus Decorated Nanobiomaterials as Scaffolds for Tissue Engineering

  • Aida Alipour Shiraz University of Medical Sciences
Keywords: Scaffolds, Tissue engineering, Virus-based nanoparticles, Nanomaterials


     One of the applications of tissue engineering is to develop artificial scaffolds. These scaffolds can mimic extracellular matrix and support cells for the repair of damaged tissue and organs. Virus particles can be re-engineering by genetic and chemical modification. Scaffolds can support cell growth and regulate cellular functions such as adhesion, spreading and proliferation. Scaffolds can be two dimensional or three dimensional which are resulting from self-assembly of the re-engineered. In this review, we review the role of virus based scaffolds in vivo and their applications in tissue engineering


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1. Mousavi, S.M., et al., A conceptual review of rhodanine: current applications of antiviral drugs, anticancer and antimicrobial activities. Artificial cells, nanomedicine, and biotechnology, 2019. 47(1): p. 1132-1148.
2. Laurencin, C.T. and L.S. Nair, Nanotechnology and tissue engineering: the scaffold. 2008: CRC Press.
3. Paul, A., et al., Nanoengineered biomimetic hydrogels for guiding human stem cell osteogenesis in three dimensional microenvironments. Journal of Materials Chemistry B, 2016. 4(20): p. 3544-3554.
4. Gholami, A., et al., 3D nanostructures for tissue engineering, cancer therapy, and gene delivery. Journal of Nanomaterials, 2020. 2020.
5. Hasan, A., et al., Nanoparticles in tissue engineering: applications, challenges and prospects. International journal of nanomedicine, 2018. 13: p. 5637.
6. Hasan, A., et al., Microfluidic techniques for development of 3D vascularized tissue. Biomaterials, 2014. 35(26): p. 7308-7325.
7. Hasan, A., et al., Biomechanical properties of native and tissue engineered heart valve constructs. Journal of biomechanics, 2014. 47(9): p. 1949-1963.
8. Luckanagul, J.A., et al., Plant virus incorporated hydrogels as scaffolds for tissue engineering possess low immunogenicity in vivo. Journal of biomedical materials research Part A, 2015. 103(3): p. 887-895.
9. Li, F. and Q. Wang, Fabrication of nanoarchitectures templated by virus‐based nanoparticles: strategies and applications. Small, 2014. 10(2): p. 230-245.
10. Manchester, M. and N.F. Steinmetz, Viruses and nanotechnology. 2009: Springer.
11. Love, A.J., et al., The use of tobacco mosaic virus and cowpea mosaic virus for the production of novel metal nanomaterials. Virology, 2014. 449: p. 133-139.
12. Raja, I.S., et al., Virus-incorporated biomimetic nanocomposites for tissue regeneration. Nanomaterials, 2019. 9(7): p. 1014.
13. Fischer, R., et al., Plant-based production of biopharmaceuticals. Current opinion in plant biology, 2004. 7(2): p. 152-158.
14. Zeng, Y., J. Hoque, and S. Varghese, Biomaterial-assisted local and systemic delivery of bioactive agents for bone repair. Acta biomaterialia, 2019. 93: p. 152-168.
15. Monteiro, N., et al., Immobilization of bioactive factor-loaded liposomes on the surface of electrospun nanofibers targeting tissue engineering. Biomaterials science, 2014. 2(9): p. 1195-1209.
16. Mehrabani, J., et al., Bioleaching of sphalerite sample from Kooshk lead–zinc tailing dam. Transactions of Nonferrous Metals Society of China, 2013. 23(12): p. 3763-3769.
17. Asghari, I. and S. Mousavi, Effects of key parameters in recycling of metals from petroleum refinery waste catalysts in bioleaching process. Reviews in Environmental Science and Bio/Technology, 2014. 13(2): p. 139-161.
18. Emami-Meibodi, M., et al., An experimental investigation of wastewater treatment using electron beam irradiation. Radiation Physics and Chemistry, 2016. 125: p. 82-87.
19. Rasoulianboroujeni, M., et al., Dual porosity protein-based scaffolds with enhanced cell infiltration and proliferation. Scientific reports, 2018. 8(1): p. 1-10.
20. Kumar, P., et al., Comprehensive survey on nanobiomaterials for bone tissue engineering applications. Nanomaterials, 2020. 10(10): p. 2019.
21. Lee, S.Y., J.S. Lim, and M.T. Harris, Synthesis and application of virus‐based hybrid nanomaterials. Biotechnology and bioengineering, 2012. 109(1): p. 16-30.
22. Deshayes, S. and R. Gref, Synthetic and bioinspired cage nanoparticles for drug delivery. Nanomedicine, 2014. 9(10): p. 1545-1564.
23. Rasoulnia, P. and S.á. Mousavi, V and Ni recovery from a vanadium-rich power plant residual ash using acid producing fungi: Aspergillus niger and Penicillium simplicissimum. RSC advances, 2016. 6(11): p. 9139-9151.
24. Mehrabani, J., S. Mousavi, and M. Noaparast, Evaluation of the replacement of NaCN with Acidithiobacillus ferrooxidans in the flotation of high-pyrite, low-grade lead–zinc ore. Separation and purification technology, 2011. 80(2): p. 202-208.
25. Kung, S.-D. and S.-F. Yang, Discoveries in plant biology. Vol. 3. 1998: World scientific.
27. Klug, A., The tobacco mosaic virus particle: structure and assembly. Philosophical Transactions of the Royal Society of London. Series B: Biological Sciences, 1999. 354(1383): p. 531-535.
28. Douglas, T. and M. Young, Host–guest encapsulation of materials by assembled virus protein cages. Nature, 1998. 393(6681): p. 152-155.
29. Douglas, T. and M. Young, Virus particles as templates for materials synthesis. Advanced Materials, 1999. 11(8): p. 679-681.
30. Ravanshad, R., et al., Application of nanoparticles in cancer detection by Raman scattering based techniques. Nano reviews & experiments, 2018. 9(1): p. 1373551.
31. Mousavi, S.M., et al., Data on cytotoxic and antibacterial activity of synthesized Fe3O4 nanoparticles using Malva sylvestris. Data in brief, 2020. 28: p. 104929.
32. Bahrani, S., et al., Zinc-based metal–organic frameworks as nontoxic and biodegradable platforms for biomedical applications: review study. Drug metabolism reviews, 2019. 51(3): p. 356-377.
33. Omidifar, N., et al., Different Laboratory Diagnosis methods of COVID-19: A Systematic Review. Archives of Clinical Infectious Diseases, 2021. 16(1).
34. Yildiz, I., S. Shukla, and N.F. Steinmetz, Applications of viral nanoparticles in medicine. Current opinion in biotechnology, 2011. 22(6): p. 901-908.
35. Mousavi, S.M., et al., Development of hydrophobic reduced graphene oxide as a new efficient approach for photochemotherapy. RSC Advances, 2020. 10(22): p. 12851-12863.
36. Avval, Z.M., et al., Introduction of magnetic and supermagnetic nanoparticles in new approach of targeting drug delivery and cancer therapy application. Drug metabolism reviews, 2020. 52(1): p. 157-184.
37. Hosseini, H., M. Kokabi, and S.M. Mousavi, Dynamic mechanical properties of bacterial cellulose nanofibres. Iranian Polymer Journal, 2018. 27(6): p. 433-443.
38. Lin, Y., et al., Layer-by-layer assembly of viral capsid for cell adhesion. Acta biomaterialia, 2008. 4(4): p. 838-843.
39. Mousavi, S.M., et al., Multifunctional Gold Nanorod for Therapeutic Applications and Pharmaceutical Delivery Considering Cellular Metabolic Responses, Oxidative Stress and Cellular Longevity. Nanomaterials, 2021. 11(7): p. 1868.
40. Hashemi, S.A., et al., Ultrasensitive Biomolecule‐Less Nanosensor Based on β‐Cyclodextrin/Quinoline Decorated Graphene Oxide toward Prompt and Differentiable Detection of Corona and Influenza Viruses. Advanced Materials Technologies, 2021: p. 2100341.
41. Bruckman, M.A., et al., Surface modification of tobacco mosaic virus with “click” chemistry. ChemBioChem, 2008. 9(4): p. 519-523.
42. Rahmani, J., et al., Elevated liver enzymes and cardiovascular mortality: a systematic review and dose–response meta-analysis of more than one million participants. European journal of gastroenterology & hepatology, 2019. 31(5): p. 555-562.
43. Hashemi, S.A., et al., Ultra-sensitive viral glycoprotein detection NanoSystem toward accurate tracing SARS-CoV-2 in biological/non-biological media. Biosensors and Bioelectronics, 2021. 171: p. 112731.
44. AndrewáLee, L., Oriented cell growth on self-assembled bacteriophage M13 thin films. Chemical Communications, 2008(41): p. 5185-5187.
45. Takmil, F., et al., Nano-magnetically modified activated carbon prepared by oak shell for treatment of wastewater containing fluoride ion. Advanced Powder Technology, 2020. 31(8): p. 3236-3245.
46. Mousavi, S.M., et al., Nanosensors for chemical and biological and medical applications. Med Chem (Los Angeles), 2018. 8(8): p. 2161-0444.1000515.
47. Kaur, G., et al., The promotion of osteoblastic differentiation of rat bone marrow stromal cells by a polyvalent plant mosaic virus. Biomaterials, 2008. 29(30): p. 4074-4081.
48. Kaur, G., et al., Regulation of osteogenic differentiation of rat bone marrow stromal cells on 2D nanorod substrates. Biomaterials, 2010. 31(7): p. 1732-1741.
49. Kaur, G., et al., The synergistic effects of multivalent ligand display and nanotopography on osteogenic differentiation of rat bone marrow stem cells. Biomaterials, 2010. 31(22): p. 5813-5824.
51. Pokorski, J.K. and N.F. Steinmetz, The art of engineering viral nanoparticles. Molecular pharmaceutics, 2011. 8(1): p. 29-43.
52. Mousavi, S.M., et al., Adsorption and removal characterization of nitrobenzene by graphene oxide coated by polythiophene nanoparticles. Physical Chemistry Research, 2020. 8(2): p. 225-240.
53. Mousavi, S.M., et al., Recent biotechnological approaches for treatment of novel COVID-19: from bench to clinical trial. Drug Metabolism Reviews, 2021. 53(1): p. 141-170.
54. Moteshafi, H., S. Mousavi, and S. Shojaosadati, The possible mechanisms involved in nanoparticles biosynthesis. Journal of Industrial and Engineering Chemistry, 2012. 18(6): p. 2046-2050.
55. Mousavi, S.M., et al., Development of clay nanoparticles toward bio and medical applications. 2018: IntechOpen.
56. Patrick, C.W., A.G. Mikos, and L.V. McIntire, Frontiers in tissue engineering. 1998: Elsevier.
57. Pittenger, M.F., et al., Multilineage potential of adult human mesenchymal stem cells. science, 1999. 284(5411): p. 143-147.
58. Caplan, A.I. and S.P. Bruder, Mesenchymal stem cells: building blocks for molecular medicine in the 21st century. Trends in molecular medicine, 2001. 7(6): p. 259-264.
59. Andersson, A.-S., et al., Influence of systematically varied nanoscale topography on the morphology of epithelial cells. IEEE transactions on nanobioscience, 2003. 2(2): p. 49-57.
60. Sniadecki, N.J., et al., Nanotechnology for cell–substrate interactions. Annals of biomedical engineering, 2006. 34(1): p. 59-74.
61. Zhao, X., Y. Lin, and Q. Wang, Virus‐based scaffolds for tissue engineering applications. Wiley Interdisciplinary Reviews: Nanomedicine and Nanobiotechnology, 2015. 7(4): p. 534-547.
62. Yu, T.T. and M.S. Shoichet, Guided cell adhesion and outgrowth in peptide-modified channels for neural tissue engineering. Biomaterials, 2005. 26(13): p. 1507-1514.
63. Mousavi, S.M., et al., Gold nanostars-diagnosis, bioimaging and biomedical applications. Drug metabolism reviews, 2020. 52(2): p. 299-318.
64. Molina, M.I.E., K.G. Malollari, and K. Komvopoulos, Design Challenges in Polymeric Scaffolds for Tissue Engineering. Frontiers in Bioengineering and Biotechnology, 2021. 9.
How to Cite
Alipour A. Virus Decorated Nanobiomaterials as Scaffolds for Tissue Engineering. AANBT [Internet]. 19Sep.2021 [cited 4Oct.2022];2(4):79-5. Available from: