Ethanolic Extract of Combined Cynodon dactylon and Mimosa pudica Ameliorated Experimentally Induced Benign Prostatic Hyperplasia in Wistar Rats

  • Adesanya Olamide Adewale Department of Anatomy, Faculty of Basic Medical Sciences, Olabisi Onabanjo University-Ikenne Campus, Ikenne, Nigeria
  • Suleiman Isa Adedeji Department of Anatomy, Faculty of Basic Medical Sciences, Olabisi Onabanjo University-Ikenne Campus, Ikenne, Nigeria
  • Odubela Olukayode Olusola Department of Anatomy, Faculty of Basic Medical Sciences, Olabisi Onabanjo University-Ikenne Campus, Ikenne, Nigeria
  • Sheu Oluwadare Sulaiman Physiology Department, Faculty of Biomedical Sciences, Kampala International University - Western Campus, P.O. Box 71, Ishaka, Uganda
  • Imade Oluwatosin Victoria Physiology Department, Faculty of Biomedical Sciences, Kampala International University - Western Campus, P.O. Box 71, Ishaka, Uganda
Keywords: Prostatic hyperplasia, Cynodon dactylon, Mimosa pudica, Prostate-specific antigen, Sperm count


Cynodon dactylon (CD) and Mimosa pudica (MP) have been known in folk medicine to relieve urinary troubles, and scientific studies have demonstrated their anti-inflammatory, diuretic, nerve regenerative, antispasmodic, pain alleviation, and muscle relaxant potentials. This study attempted to screen extract of CD and MP combination (CDMP) for its ability to ameliorate experimentally induced prostatic hyperplasia in the rat model, which could serve as possible management of benign prostatic hyperplasia (BPH). Twenty adult male Wistar rats were divided randomly into four groups. Groups A (normal control) and B (induced BPH control) received oral administration of distilled water, while IBPH groups C, and D received oral administration of low dose (LD) 0.46 g/ml and high dose (HD) 0.92 g/ml of CDMP extract per Kg BW, respectively for four weeks. Blood samples were collected from each rat to determine prostate-specific antigen (PSA) concentrations. Caudal epididymal sperm count, and testicular weight and histological examination were also evaluated. The CDMP ethanolic extract caused a significant (p<0.05) reduction in PSA levels and relative weight of the prostate in the treated rats. The relative weight of testis was significantly higher in CDMP treated groups. The sperm count in all IBPH groups was significantly (p<0.05) reduced. Evidence of recoveries was observed in prostates of CDMP treated groups with an increase in the fibromuscular, interacinar tissue, thinning of the prostate epithelium, and stroma. The findings suggest that ethanolic extracts of Cynodon dactylon and Mimosa pudica can serve as a potential candidate in the management of benign prostatic hyperplasia and its symptoms.


Saito M, Tsounapi P, Oikawa R, et al. Prostatic ischemia induces ventral prostatic hyperplasia in the SHR; possible mechanism of development of BPH. Scientific Reports. 2014;4:3822.1-7.

Bostanci Y, Kazzazi A, Momtahen S, et al. Correlation between benign prostatic hyperplasia and inflammation. Current Opinion in Urology. 2013;23:5-10

Sáez C, González‐Baena AC, Japón MA, et al. Regressive changes in fnasteride-treated human hyperplastic prostates correlate with an upregulation of TGF-beta receptor expression. Prostate. 2011;37:84-90.

Tract Symptoms/Benign Prostatic Hyperplasia Guidelines Committee. Singapore urological association clinical guidelines for male lower urinary tract symptoms/benign prostatic hyperplasia. Singapore medical journal. 2017;58(8):473-480.

Kim HK, Zhao C, Choi BR, et al. Is transforming growth factor-beta signaling activated in human hypertrophied prostate treated by 5-alpha reductase inhibitor? Disease Markers. 2013;35( 6):679-685.

Minutoli L, Rinaldi M, Marini H. et al. Apoptotic pathways linked to endocrine system as potential therapeutic targets for benign prostatic hyperplasia. International Journal of Molecular Sciences. 2016;17:1311.

Arora RP, Nayak RL, Malhotra V, Mohanty NK. Role of herbal drugs in the management of benign prostatic hyperplasia: Clinical trial to evaluate the efficacy and safety of Himplasia. Med Update. 2003;11:55-58.

Caesar LK, Cech NB. Synergy and antagonism in natural product extracts: when 1+ 1 does not equal 2. Natural product reports. 2019;36(6):869-888.

Adesanya AO, Olaseinde OO, Oguntayo OD, et al. Effects of Methanolic Extract of Citrullus lanatus Seed on Experimentally Induced Prostatic Hyperplasia. European Journal of Medicinal Plants. 2011;1(4):171-179.

KOBASI H. Dictionary of Herbs and Medicinal plants. Scientific Books House, Beirut, Lebanon, (1993):Pp 566.

Rizk AM, El-ghazaly GA. Medicinal and poisonous plants of Qatar. Scientific and applied research centre, university of Qatar. 1995;50-150.

Boulos L. Medicinal plants of North Africa. Reference publication Inc. Algonac, Michigan USA (1983).

Al-Rawi A, Chakravarty HL. Medicinal plants of Iraq. National herbarium of Iraq. Ministry of agriculture. Baghdad-Iraq. 1964.

Duke JA, Wain KK. Medicinal plants of the world. Computer index with more than 85000 entries. Handbook of Medicinal Herbs. 1981:96.

Ahmad H, Sehgal S, Mishra A, Gupta R. Mimosa pudica L.(Laajvanti): an overview. Pharmacognosy reviews. 2012;6(12):115.

Muhammad G, Hussain MA, Jantan I. A high‐value medicinal plant as a source of bioactives for pharmaceuticals. Comprehensive Reviews in Food Science and Food Safety. 2016;15(2):303-315.

Thompson IM. : Pharmacologic agents in complementary medicine in prostatic. Drugs Today. 2001;37:427-433.

Roehrborn CG. The epidemiology of acute urinary retention in benign prostatic hyperplasia. Reviews in urology. 2001;3(4):187.

Nozhat F, Alaee S, Behzadi Kh, Azadi Chegini N. Evaluation the possible toxic effects of spearmint (Mentha spicata) on the reproductive system, fertility and number of offspring of adult male rats. Avicenna Journal of Phytomedicine. 2014;4(6):420-429.

Adesanya OA, Shittu LAJ, Awobajo FO, et al. Knowledge and Practice of Prostate Health of Men in the Work Place. Journal of Infertility and Reproductive Biology. 2013;1(2):54-57.

Pais P. Potency of a novel saw palmetto extract, SPET-085, for inhibition of 5 alpha-reductase II. Advances in Therapy. 2010;27:555-563.

Arruzazabala ML, Ma R, Molina V, et al. Effect of D-004, alipid extract from the fruits of Cuban royal palm, on the atypical prostate hyperplasia induced with phenylephrine in rats. Drugs in R&D. 2006;7:233-241.

Veeresh BSV, Veeresh B, Patill AA, et al. Lauric acid and myristic acid prevent testosterone induced prostatic hyperplasia in rats. European Journal of Pharmacology. 2010;625:262-265.

Bent S, Kane C, Shinohara K, et al. Saw Palmetto for Benign Prostatic Hyperplasia. New England Journal of Medicine. 2006;354:557-566.

Creasy DM, Chapin RE. Male reproductive system. Haschek and Rousseaux's handbook of Toxicologic Pathology. 2013;1:2493-598.

Wang C, Swerdloff RS. Hormonal Approaches to Male contraception. Current Opinion Urology. 2010;20(6):520-524.

Shin IS, Lee MY, Ha HK, et al. Inhibitory effect of Yukmijihwang-tang, a traditional herbal formula against testosterone-induced benign prostatic hyperplasia in rats. BMC Complementary and Alternative Medicine. 2012;12(1):1-7.

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