Attenuating effect of polyalthia longifolia on cadmium sulfate- induced testicular toxicity

  • Ogunbiyi Olubunmi E Department of Anatomy, Faculty of Basic Medical Sciences, Colleges of Health Sciences, Babcock University, IIishan-Remo, Nigeria
  • Oyewopo Adeoye Oyetunji Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, University of Ilorin, Ilorin, Nigeria
  • Tokunbo Olorunfemi S Department of Anatomy, Faculty of Basic Medical Sciences, Osun State University, Osogbo, Nigeria
  • Adeleke Opeyemi Samson Department of Anatomy, Faculty of Basic Medical Sciences, Osun State University, Osogbo, Nigeria
  • Abayomi Taiwo Adekemi Department of Anatomy, Faculty of Basic Medical Sciences, Osun State University, Osogbo, Nigeria
  • Johnson Olawumi Feyisike Department of Anatomy, Faculty of Basic Medical Sciences, College of Health Sciences, University of Ilorin, Ilorin, Nigeria
  • Bello Matthew K. Department of Pharmacology and Therapeutics, Faculty of Basic Medical Sciences, University of Ilorin, Nigeria
Keywords: Cadmium Sulfate, Testis, Histology, Immunohistochemistry, Semen analysis


Polyalthia longifolia extract has numerous beneficial effects on human health. They have been reported to have antiviral, anti-allergic, anti-inflammatory, anti-tumour and antioxidant activities. Cadmium is used in the production of nickel cadmium batteries, pigments ceramics, plastic stabilizers, and fertilizers. Cigarette smoke is one of the most important sources of cadmium exposure in the general non-occupationally exposed population. In nonsmokers, the main source of cadmium is food, particularly cereals such as rice and wheat, green leafy vegetables, potato, and offal products such as liver and kidney. Twenty male Wistar rats were divided into four groups. Group A were orally treated with distilled water for twenty eight days, Group B were intraperitoneal treated with cadmium sulfate for three days and sacrificed twenty four hours later, Group C received oral administration of Polyalthia longifolia for twenty eight days and Group D received cadmium sulfate intraperitoneally for three days and post treated orally with Polyalthia longifolia for twenty five days. On the 29th day the animals were sacrificed through cervical dislocation, the right testis was excised and processed histologically, while the left testis was processed for semen analysis. The histological observation showed that Polyalthia longifolia administration improved the histoarchitectural distortion of the testis in the cadmium sulphate treated group. Polyalthia longifolia administration restored the alteration on the sperm parameters of the damaged testes. The result suggested that Polyalthia longifolia restored testicular toxicity induced by cadmium sulphate and may be used as sub fertility drug.


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Balen AH, Rutherford AJ. Management of infertility. Bmj. 2007;335(7620):608-611.

Arcaniolo D, Favilla V, Tiscione D, et al. Is there a place for nutritional supplements in the treatment of idiopathic male infertility? Archivio Italiano di Urologia e Andrologia. 2014;86(3):164-170.

Levine H, Jørgensen N, Martino-Andrade A, et al. Temporal trends in sperm count: a systematic review and meta-regression analysis. Human reproduction update. 2017;23(6):646-659.

Jenardhanan P, Panneerselvam M, Mathur PP, editors. Effect of environmental contaminants on spermatogenesis. Seminars in cell and developmental biology. 2016: 59.

Akinloye O, Arowojolu AO, Shittu OB, Anetor JI. Cadmium toxicity: a possible cause of male infertility in Nigeria. Reproductive Biology. 2006;6(1):17-30.

Fay R, Mumtaz M. Development of a priority list of chemical mixtures occurring at 1188 hazardous waste sites, using the HazDat database. Food and chemical toxicology. 1996;34(11-12):1163-1165.

Organization WH. Biomarkers in Risk Assessment: Validity and Vlidation-Environmental Health Criteria 2222001.

Soleimani A. Environmental Pollution: a Risk Factor for Female Fertility-A Letter to Editor. Journal of Infertility and Reproductive Biology. 2020;8(4):66-67.

Carette D, Perrard M-H, Prisant N, Gilleron J, Pointis G, et al. Hexavalent chromium at low concentration alters Sertoli cell barrier and connexin 43 gap junction but not claudin-11 and N-cadherin in the rat seminiferous tubule culture model. Toxicology and applied pharmacology. 2013;268(1):27-36.

de Angelis C, Galdiero M, Pivonello C, et al. The environment and male reproduction: the effect of cadmium exposure on reproductive function and its implication in fertility. Reproductive Toxicology. 2017;73:105-127.

Malairajan P, Gopalakrishnan G, Narasimhan S, Veni KJK. Analgesic activity of some Indian medicinal plants. Journal of ethnopharmacology. 2006;106(3):425-428.

Saleem R, Ahmed M, Ahmed SI, et al. Hypotensive activity and toxicology of constituents from root bark of Polyalthia longifolia var. pendula. Phytotherapy Research: An International Journal Devoted to Pharmacological and Toxicological Evaluation of Natural Product Derivatives. 2005;19(10):881-884.

Nair R, Shukla V, Chanda S. Assessment of Polyalthia longifolia var. pendula for hypoglycemic and antihyperglycemic activity. Journal of Clinical and Diagnostic Research. 2007;1:116-121.

Malairajan P, Gopalakrishnan G, Narasimhan S, Veni KJK. Evalution of anti-ulcer activity of Polyalthia longifolia (Sonn.) Thwaites in experimental animals. Indian journal of pharmacology. 2008;40(3):126.

Marthanda Murthy M. Annapurna J: Antibacterial activity of Clerodane diterpenoids from Polyalthia longifolia seeds. Fitoterapia. 2005;76:3-4.

Faizi S, Khan RA, Mughal NR, et al. Antimicrobial activity of various parts of Polyalthia longifolia var. pendula: isolation of active principles from the leaves and the berries. Phytotherapy Research. 2008;22(7):907-912.

Chang F-R, Hwang T-L, Yang Y-L, et al. Anti-inflammatory and cytotoxic diterpenes from formosan Polyalthia longifolia var. pendula. Planta medica. 2006;72(14):1344-1347.

Ukwenya V, Ashaolu J, Adeyemi A, et al. Antihyperglycemic activities of methanolic leaf extract of Anacardium occidentale (Linn.) on the pancreas of streptozotocin-induced diabetic rats. Journal of Cell and Animal Biology. 2012;6(11):169-174.

Drury RAB, Wallington EA, Cameron SR. Carleton's histological technique: London; 1967.

Adamkovicova M, Toman R, Cabaj M, et al. Effects of subchronic exposure to cadmium and diazinon on testis and epididymis in rats. The Scientific World Journal. 2014;2014.

Alaee S, Talaiekhozani A, Rezaei S, et al. Cadmium and male infertility. Journal of Infertility and Reproductive Biology. 2014;2(2):62-69.

Ogunbinu AO, Ogunwande IA, Essien E, Cioni PL, Flamini G. Sesquiterpenes-rich essential oils of Polyalthia longifolia Thw.(Annonaceae) from Nigeria. Journal of Essential Oil Research. 2007;19(5):419-421.

Kalwar Q, Chu M, Ahmad AA, et al. Morphometric evaluation of spermatogenic cells and seminiferous tubules and exploration of luteinizing hormone beta polypeptide in testis of datong yak. Animals. 2020;10(1):66.

Lestari SW, Aditya D, Pratama G, et al. A modification of Johnson score as predictive value of sperm retrieval in non-obstructive azoospermia infertile men. AIP Conference Proceedings; 2019: AIP Publishing LLC.

Shaikh ZA, Zaman K, Tang W, Vu T. Treatment of chronic cadmium nephrotoxicity by N-acetyl cysteine. Toxicology letters. 1999;104(1-2):137-142.

El-Demerdash FM, Yousef MI, Kedwany FS, Baghdadi HH. Cadmium-induced changes in lipid peroxidation, blood hematology, biochemical parameters and semen quality of male rats: protective role of vitamin E and β-carotene. Food and chemical toxicology. 2004;42(10):1563-1571.

Yang J-M, Arnush M, Chen Q-Y, et al. Cadmium-induced damage to primary cultures of rat Leydig cells. Reproductive toxicology. 2003;17(5):553-560.

Theocharis SE, Margeli AP, Ghiconti IK, Varonos D. Liver thymidine kinase activity after cadmium-induced hepatotoxicity in rats. Toxicology letters. 1992;63(2):181-190.

Wu T-H, Cheng Y-Y, Chen C-J, et al. Three new clerodane diterpenes from Polyalthia longifolia var. pendula. Molecules. 2014;19(2):2049-2060.

Jurikova M, Danihel Ľ, Polák Š, et al. KI67, PCNA and MCM proteins: Markers of proliferation in the diagnosis of breast cancer. Acta histochemica. 2016;118(5):544-552.

Chang H-L, Chang F-R, Chen J-S, et al. Inhibitory effects of 16-hydroxycleroda-3, 13 (14) E-dien-15-oic acid on superoxide anion and elastase release in human neutrophils through multiple mechanisms. European journal of pharmacology. 2008;586(1-3):332-339.

Birnbaum L, Korach K. Reproductive and Developmental Toxicology. 1998.

Pasqualotto FF, Locambo CV, Athayde KS, Arap S. Measuring male infertility: epidemiological aspects. Revista do Hospital das Clínicas. 2003;58(3):173-178.

Aydos K, Güven M, Can B, Ergün A. Nicotine toxicity to the ultrastructure of the testis in rats. BJU international. 2001;88(6):622-626.

Khaki A, Ouladsahebmadarek E, Javadi L, et al. Anti-oxidative effects of citro flavonoids on spermatogenesis in rat. African Journal of Pharmacy and Pharmacology. 2011;5(6):721-725.

Hall JE, Hall ME. Guyton and Hall textbook of medical physiology e-Book: Elsevier Health Sciences; 2020.

How to Cite
Olubunmi E, O., Adeoye Oyetunji, O., Olorunfemi S, T., Opeyemi Samson, A., Taiwo Adekemi, A., Olawumi Feyisike, J., & Matthew K., B. (2021). Attenuating effect of polyalthia longifolia on cadmium sulfate- induced testicular toxicity. Journal of Infertility and Reproductive Biology, 9(2), 52-58.
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