Prevention of Arsenic Induced Testicular Oxidative Stress and DNA Damage by Coenzyme Q10 and Vitamin E in Swiss Albino Mice

  • Anupama Sharma Division of Reproductive and Cytotoxicology, ICMR-National Institute of Occupational Health, Ahmedabad-380016, India
  • Chaoba Kshetriyanum Division of Reproductive and Cytotoxicology, ICMR-National Institute of Occupational Health, Ahmedabad-380016, India
  • Bharat Patel Division of Reproductive and Cytotoxicology, ICMR-National Institute of Occupational Health, Ahmedabad-380016, India
  • Rekha Kashyap Former Scientist F, ICMR-National Institute of Occupational Health, Ahmedabad-380016, India
  • Harsiddha G Sadhu Former Scientist F, ICMR-National Institute of Occupational Health, Ahmedabad-380016, India
  • Sunil Kumar Former Scientist G & Director I/C, ICMR-NIOH, Division of Reproductive and Cytotoxicology, ICMR-National Institute of Occupational Health, Ahmedabad-380016, India
Keywords: Arsenic, Vitamin E, Testis, Coenzyme Q10, DNA damage

Abstract

Arsenic toxicity has become one of the major public health problems in certain parts of the world. Thus, it is rational to find out a suitable compound to prevent arsenic-induced toxicity for clinical usage. Hence, the Coenzyme Q10 and Vitamin E were tested against arsenic-induced testicular oxidative stress and DNA damage. The mice were divided into five groups, animals of the four groups were exposed to 136 ppm arsenic via drinking water for 30 days. Subsequently, animals of three groups were treated with Vitamin E (50 mg/kg b.wt.), Coenzyme Q10 (10 mg/kg b.wt.), and their combination for 30 days, and animals of the 4th group were maintained without antioxidant treatment. The animals of the 5th group (without any treatment) served as control. Thereafter, blood was collected, for DNA damage study, and testis dissected out to assess oxidative stress. The body and testis weight gain were lower in the arsenic subjected group compared to the control group whereas antioxidants (Vitamin E, Coenzyme Q10, and combination) treatment checks to some extent this decline. Biochemical data indicated that lipid peroxidation level was higher while reduced glutathione, total thiol, superoxide dismutase, and total protein level was significantly lesser in the arsenic exposed group compared to the control group, and antioxidants treatment diminished arsenic-induced these alterations to some extent. Arsenic induces DNA damage in the blood cells of mice by displaying a significantly lower head DNA percentage and a higher level of tail DNA percentage, tail length, tail moment, while Vitamin E, Coenzyme Q10, and combination were able to lower these changes. The data further revealed that the combined treatment of Vitamin E, Coenzyme Q10 is more effective than the treatment of these antioxidants individually.

Downloads

Download data is not yet available.

References

WHO. 2018.Arsenic, Key facts. https://www.who.int/news-room/fact-sheets/detail/ arsenic.Retrieved on 29-4-2021.

Sharma A, Kumar S. Arsenic exposure with reference to neurological impairment: An overview. Reviews on Environmental Health. 2019;34(4):403-414. doi: 10.1515/reveh-2019-0052.

Ravenscroft P, Brammer H, Richards K. Arsenic pollution: a global synthesis. John Wiley & Sons; 2008 pp 28.

Wang A, Holladay SD, Wolf DC. Reproductive and developmental toxicity of arsenic in rodents: a review. International Journal of Toxicology. 2006;25(5): 319-331. doi: 10.1080/10915810600840776.

Kim YJ, Kim JM. Arsenic toxicity in male reproduction and development. Development Reproduction. 2015;19(4):167-180. doi: 10.12717/DR.2015.19.4.167

Pant N, Murthy RC, Srivastava SP. Male reproductive toxicity of sodium arsenite in mice. Human and Experimental Toxicology. 2004;23(8):399-403. doi: 10.1191/0960327104ht467oa.

Ali M, Khan SA, Dubey P, et al. Impact of arsenic on testosterone synthesis pathway and sperm production in mice. Innovative Journal of Medical and Health Science. 2013;3(4):185-189.

Muenyi CS, Ljungman M. Arsenic disruption of DNA damage responses—potential role in carcinogenesis and chemotherapy. Biomolecules. 2015;5(4):2184-2193. doi: 10.3390/biom5042184

Bhattacharya S. Medicinal plants & natural products in amelioration of arsenic toxicity: a short review. Pharmaceutical Biology. 2017;55(1):349-354. doi: 10.1080/13880209.2016.1235207.

Bentinger M, Brismar K, Dallner G. The antioxidant role of coenzyme Q. Mitochondrion. 2007; 7: S41-S51. doi:10.1016/j.mito.2007.02.006.

Crane FL. Biochemical functions of coenzyme Q10. Journal of the American College of Nutrition.. 2001; 20(6):591-598. doi: 10.1080/07315724.2001.10719063.

Paunović MG, Matić MM, Ognjanović BI, Saičić ZS. Antioxidative and haematoprotective activity of coenzyme Q10 and Vitamin E against cadmium-induced oxidative stress in Wistar rats. Toxicology Industrial Health. 2017; 33(10): 746-756. doi: 10.1177/0748233717725480

Ramadan LA, Abd-Allah AR, Aly HA, Saad-El-Din AA. Testicular toxicity effects of magnetic field exposure and prophylactic role of coenzyme Q10 and L-carnitine in mice. Pharmacology Research. 2002;46(4):363-370. doi: 10.1016/s1043661802001718.

Beyer RE. The role of ascorbate in antioxidant protection of biomembranes: interaction with Vitamin E and Coenzyme Q10. Journal of Bioenergetics and Biomembrane.1994;26(4): 349-358. doi: 10.1007/BF00762775.

Chen H, Tappel AL. Protection of Vitamin E, selenium, trolox C, ascorbic acid palmitate, acetylcysteine, coenzyme Q0, coenzyme Q10, beta-carotene, canthaxanthin, & (+)-catech in against oxidative damage to rat blood and tissues in vivo. Free Radical Biology and Medicine. 1995;18(5):949-953. doi: 10.1016/0891-5849(94)00238-f.

Ibrahim WH, Bhagavan HN, Chopra RK, Chow CK. Dietary coenzyme Q10 & Vitamin E alter the status of these compounds in rat tissues & mitochondria. The Journal of Nutrition. 2000; 130(9):2343-2348. doi: 10.1093/jn/130.9.2343.

Ognjanović BI, Marković SD, Ðorđević NZ, et al. Cadmium-induced lipid peroxidation and changes in antioxidant defense system in the rat testes: Protective role of coenzyme Q10 & Vitamin E. Reproductive Toxicology. 2010;29(2):191-197. doi: 10.3390/antiox9060492.

Sharma A, Chaoba Ksh, Sadhu HG, Kumar S. Arsenic-induced oxidative stress, cholinesterase activity in the brain of Swiss albino mice, and its amelioration by antioxidants Vitamin E and Coenzyme Q10. Environmental Science Pollution Research. 2018;25(24):23946-23953. doi: 10.1007/s11356-018-2398-z.

Buege JA, Aust SD. In Methods in Enzymology (eds) Microsomal lipid peroxidation. Academic Press. 1978; pp302-310.

Ellman GL. Tissue sulfhydryl groups. Archives of Biochemistry and Biophysics. 1959;82(1):70-77. doi: 10.1016/0003-9861(59)90090-6.

Hu ML In Methods in enzymology (eds) Measurement of protein thiol groups and glutathione in plasma. Academic Press. 1994; pp 380-385.

Marklund S, Marklund G. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. European Journal of Biochemistry. 1974; 47(3): 469-474. doi: 10.1111/j.1432-1033.1974.tb03714.x.

Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry. 1951; 193:265-275.

Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research. 1988; 175 (1): 184-191. doi: 10.1016/0014-4827(88)90265-0

Chang SI, Jin B, Youn P, et al. Arsenic-induced toxicity and the protective role of ascorbic acid in mouse testis. Toxicology Applied Pharmacology.2007; 218(2):196-203. doi: 10.1016/j.taap.2006.11.009.

Neiger RD, Osweiler GD. Arsenic concentrations in tissues and body fluids of dogs on chronic low-level dietary sodium arsenite. Journal of Veterinary Diagnostic Investigation. 1992;4(3): 334-337. doi: 10.1177/104063879200400318.

Maharjan M, Watanabe C, Ahmad SA, et al. Mutual interaction between nutritional status and chronic arsenic toxicity due to groundwater contamination in an area of Terai, lowland Nepal. Journal of Epidemiology Community Health. 2007;61(5):389-394. doi: 10.1136/jech.2005.045062.

Zeng Q, Yi H, Huang L,An Q, Wang H. Long-term arsenite exposure induces testicular toxicity by redox imbalance, G2/M cell arrest and apoptosis in mice. Toxicology. 2019;411:122-132. doi: 10.1016/j.tox.2018.09.010.

Mukherjee S, Roy M, Dey S, Bhattacharya RK.A mechanistic approach for modulation of arsenic toxicity in human lymphocytes by curcumin, an active constituent of medicinal herb Curcuma longa Linn. Journal of Clinical Biochemistry and Nutrition.2007;41(1):32-42. doi: 10.3164/jcbn.2007005.

Guvvala PR, Sellappan S, Parameswaraiah RJ. Impact of arsenic (V) on testicular oxidative stress and sperm functional attributes in Swiss albino mice. Environmental Science Pollution Research. 2016;23(18):18200-18210. doi: 10.1007/s11356-016-6870-3.

Li SG, Ding Yu S, Niu Q, Xu S Zhi, Pang Li J, MA Ru-L, Jing M Xia, Feng GL, Liu JM, Guo SX. Grape seed Proanthocyanid in extract alleviates arsenic-induced oxidative reproductive toxicity in male mice. Biomedical and Environmental Science, 2015:28(4):272-280. doi: 10.3967/bes2015.038.

Guillamet E, Creus A, Ponti J, Sabbioni E, Fortaner S, Marcos R. In vitro DNA damage by arsenic compounds in a human lymphoblastoid cell line (TK6) assessed by the alkaline Comet assay. Mutagenesis. 2004;19(2):129-135. doi: 10.1093/mutage/geh005.

Biswas J, Sinha D, Mukherjee S, Roy S, Siddiqi M, Roy M. Curcumin protects DNA damage in a chronically arsenic-exposed population of West Bengal. Human Experimental Toxicology. 2010;29(6):513-524. doi: 10.1177/0960327109359020.

Yamauchi H, Aminaka Y, Yoshida K, Sun G, Pi J, P Waalkes MP. Evaluation of DNA damage in patients with arsenic poisoning: urinary 8-hydroxy deoxy guanine.Toxicology Applied Pharmacology. 2004;198(3):291-296. doi: 10.1016/j.taap.2003.10.021.

Fouad AA, Al-Sultan, AI, Yacoubi MT. Coenzyme Q10 counteracts testicular injury induced by sodium arsenite in rats. European Journal of Pharmacology. 2011;655(1):91-98. doi: 10.1016/j.ejphar.2010.12.045.

Momeni HR, Eskandari N. Effect of Vitamin E on sperm parameters & DNA integrity in sodium arsenite-treated rats. Iranian Journal of Reproductive Medicine. 2012;10(3): 249-256.

Momeni HR, Eskandari N. Curcumin inhibits the adverse effects of sodium arsenite in mouse epididymal sperm. International Journal of Fertility and Sterility. 2016;10(2):245-252.

Published
2021-06-20
How to Cite
Sharma, A., Kshetriyanum, C., Patel, B., Kashyap, R., Sadhu, H., & Kumar, S. (2021). Prevention of Arsenic Induced Testicular Oxidative Stress and DNA Damage by Coenzyme Q10 and Vitamin E in Swiss Albino Mice. Journal of Infertility and Reproductive Biology, 9(2), 85-91. Retrieved from https://www.dormaj.org/index.php/JIRB/article/view/392
Section
Regular publication process (free of charge)