Fertility Assessment of Male Wistar Rats Orally Exposed to Chloroform Stem Extract of Portulaca Oleracea Linn. (Purslane)– An Experimental Study
Portulaca oleracea Linn. has a history of extensive use as a medicinal plant and is frequently used by traditional medicine practitioners in Nigeria. Different parts of P. oleracea L. have been studied for their effect on the reproductive physiology of animals but there is a paucity of information on the stem as it relates to male fertility. This study, therefore, investigated the sub-chronic effect of oral administration of P.oleracea stem extract on male fertility indices (semen analysis, testosterone concentration, and testicular histology) in Wistar rats. Twenty animals were randomly divided into 4 groups of 5 rats each. Group A(Control) received 0.5 ml of olive oil(vehicle) while Groups B, C & D received 125, 250 & 500 mg/kg of extract respectively for 21 days by oral gavage. In the end, the animals were anesthetized and blood samples were collected for testosterone assay, caudal epididymis for semen analysis, and testes for histology. P.oleracea stem extract had no significant (p>0.05) effect on serum testosterone concentration, testicular histoarchitecture, and sperm viability, morphology & motility relative to control. However, there was a significant (p=0.014) reduction in the sperm cell count of rats exposed to the highest dose (500mg/kg) in relation to the control. Oral administration of P.oleracea stem extract as used in this study may harm male fertility; thus further study is recommended to ascertain if prolonged exposure will validate this finding.
Raji Y, Morakinyo AO, Oloyo AK, et al. Impact of the chloroform extract of Carica papaya seed on oestrous cycle and fertility in female albino rats. Journal of Medical Sciences. 2005;5(4):337-343.
Bala K, Arya M, Katare DP. Herbal Contraceptive : An Overview. World Journal of Pharmacy and Pharmaceutical Sciences. 2016;3(8):1305-1326.
Saalu LC. Nigerian Folklore Medicinal Plants with Potential Antifertility Activity in Males : A Scientific Appraisal. Research Journal of Medicinal Plants. 2016; 10(3):201-227.
Mitich LW. Common Purslane (Portulaca oleracea). Weed Technology. 1997;11(2):394-397.
Obinna VC, Kagbo HD, Agu GO. Lipophilic and hydrophilic leaf extracts of Portulaca oleracea (Purslane) disrupts female sex hormones in albino rats (Rattus norvegicus). Journal of Traditional and Complementary Medicine. 2021;11(2):82-89.
Obinna V, Kagbo H, Agu G. Effects of Lipophilic and Hydrophilic leaf extracts of Portulaca oleracea Linn. (Purslane) on male reproductive parameters in albino rats. American Journal of Physiology, Biochemistry and Pharmacology. 2019;9(1):21-32.
Lorke D. A new approach to practical acute toxicity testing. Archives of Toxicology. 1983;54(4):275-287.
Arifin WN, Zahiruddin WM. Sample Size Calculation in Animal Studies Using Resource Equation Approach. Malysian Journal of Medical Sciences. 2017;24(5):101-105.
Ilyas MN, Adzim MKR, Simbak NB, A. B. Atif. Sample Size Calculation for Animal Studies Using Degree of Freedom ( E ); an Easy and Statistically Defined Approach for Metabolomics and Genetic Research. Current Trends in Biomedical Engineering and Biosciences. 2017;10(2):47-48.
Kilkenny C, Browne WJ, Cuthill IC, et al. Improving Bioscience Research Reporting : The ARRIVE Guidelines for Reporting Animal Research. PLoS Biology. 2010;8(6):6-10.
Cheesebrough M. District Laboratory Practice in tropical countries. Cambridge: Cambridge University Press; 2006. 131-132.
Lillie RD. Histopathologic technique and practice histochemistry. 3rd ed. New York: McGraw Hill Book co.; 1965.
Chinedu E, Arome D, Ameh SF. Phytochemical evaluation of the ethanolic extracts of some Nigerian herbal plants. Drug Development and Therapeutics. 2015;6(1):11-14.
Koche D, Shirsat R. Phytochemical screening of eight traditionally used ethnomedicinal plants from Akola district (MS) India. International Journal of Pharma and Bio Sciences. 2010;1(4):253-256.
Ezeabara AC, Ikeh CF, Ilodibia CV, et al. Comparative Determination of Phytochemical, Proximate and Mineral Compositions in Various Parts of Portulaca oleracea L. Journal of Plant Sciences. 2014;2(6):293-298.
Zhou YX, Xin HL, Rahman K, et al. Portulaca oleracea L.: A review of phytochemistry and pharmacological effects. BioMed Research International. 2015;2015:1-11.
Okafor IA, Nnamah SU, Ahiatrogah S, et al. Reproductive toxicity potentials of methanolic extract of Portulaca oleracea in male rats : An experimental study. International Journal of Reproductive BioMedicine. 2021;19(3):245-254.
Nayaka HB, Londonkar RL, Andumesh MK. Evaluation of portulaca oleracea l for anti-fertility effect in female albino rats. International Journal of Pharmacy and Pharmaceutical Sciences. 2014;6(5):86-89.
Oyedeji KO, Bolarinwa AF. Evaluation of the reproductive functions of Portulaca Oleracea extracts in female albino rats. Research Journal of Pharmacology. 2010;13(3):213-218.
Oyedeji KO, Bolarinwa AF. Effects Of Crude Extracts of Portulaca oleracea on Male Reproductive Functions In Albino Rats. African Journal of biomedical Research. 2013;4(6):71-79.
Okafor IA, Nnamah US, Nnaka J. The fertility assessment of normal cyclic Wistar rats following the administration of methanolic extract of Portulaca oleracea : an experimental study. Middle East Fertility Society Journal. 2021;26(5):1-10.
Copyright (c) 2023 Journal of Infertility and Reproductive Biology
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.